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Exploring the Versatility and Safety of EDC HCL: A Comprehensive Guide

EDC HCL Properties

Quality Level200
reaction suitabilityreagent type: cross-linking reagent reaction type: Peptide Synthesis
mp110-115 °C (lit.)
solubilityH2O: ≤100 mg/mL
storage temp.−20°C


EDC HCL Introduction:

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, commonly known as EDC or EDAC, is a versatile reagent extensively used in peptide chemistry, bioconjugation, and molecular biology. In this comprehensive guide, we delve into the properties, applications, safety considerations, and best practices associated with this essential compound.


  1. Peptide Synthesis: EDC is commonly used as a coupling reagent in peptide synthesis to activate carboxyl groups for amide bond formation between amino acids.
  2. Bioconjugation: It serves as a cross-linking agent in the conjugation of proteins, peptides, and nucleic acids to various molecules, such as fluorophores, antibodies, and solid supports.
  3. Surface Modification: EDC facilitates the covalent attachment of biomolecules to surfaces, enabling the immobilization of ligands, enzymes, or antibodies for biosensor applications.
  4. Drug Delivery: In pharmaceutical research, EDC is utilized in the synthesis of prodrugs and drug conjugates for targeted drug delivery systems.

Edc Hcl Structure


Biochemical and Physiological Actions:

EDC functions as a zero-length cross-linker, facilitating the formation of stable amide bonds between carboxyl and amino groups. It activates carboxyl groups by forming reactive O-acylisourea intermediates, which subsequently react with primary amines to generate stable amide linkages.

Safety Considerations:


Hazardous Properties: EDC is corrosive and may cause skin, eye, and respiratory tract irritation upon contact. It should be handled with caution, and appropriate personal protective equipment (PPE) must be worn.

Storage: EDC should be stored in a cool, dry place away from heat, moisture, and incompatible substances. Proper ventilation is necessary to prevent the accumulation of vapors.

Disposal: Disposal of EDC should be carried out in accordance with local regulations and guidelines for hazardous waste disposal. Neutralization and dilution with water before disposal are recommended.



N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, or EDC, is a vital reagent in peptide synthesis, bioconjugation, and molecular biology applications. Its versatile functionality and broad compatibility make it indispensable in various research and industrial settings. By adhering to safety protocols and best practices, researchers can harness the power of EDC to advance scientific discovery and innovation.

This guide serves as a comprehensive resource for understanding the properties, applications, and safety considerations associated with EDC, empowering researchers to utilize this essential reagent effectively and responsibly in their work.


More Product API


Safety Information:



PictogramsSkull and crossbones Health hazard Environment
Signal WordDanger

Hazard Statements

H302 – H311 – H315 – H317 – H373 – H410

Precautionary Statements

P260 – P273 – P280 – P301 + P312 – P302 + P352 + P312 – P314

Hazard Classifications

Acute Tox. 3 Dermal – Acute Tox. 4 Oral – Aquatic Acute 1 – Aquatic Chronic 1 – Skin Irrit. 2 – Skin Sens. 1 – STOT RE 2 Oral

Storage Class Code

6.1C – Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects





1. What is EDC HCL?

EDC HCL, or Ethylene Dichloride Hydrochloride, is a chemical compound used primarily in industrial processes. It is a clear, colorless liquid with a pungent odor.


2. Is EDC HCL hazardous?

Yes, EDC HCL is considered hazardous due to its toxic and flammable properties. Proper safety precautions should be followed when handling and storing this chemical to minimize risks to health and the environment.


3. What safety measures should be taken when working with EDC HCL?

When working with EDC HCL, it is essential to wear appropriate personal protective equipment (PPE), including gloves, goggles, and protective clothing, to prevent contact with skin, eyes, and clothing. Additionally, work should be conducted in a well-ventilated area to minimize exposure to vapors.


4. How should EDC HCL be stored?

EDC HCL should be stored in a cool, dry, and well-ventilated area away from heat, sources of ignition, and incompatible materials. Proper labeling and segregation from other chemicals are necessary to prevent accidental exposure or contamination.


5. Can EDC HCL be used in pharmaceutical applications?

While EDC HCL is primarily used in industrial processes, its application in pharmaceuticals is limited. It is not commonly used directly in pharmaceutical formulations due to its toxic nature.


6. What are the environmental considerations associated with EDC HCL?

EDC HCL can have adverse effects on the environment if released into water bodies or soil. Proper disposal practices should be followed to prevent contamination and minimize environmental impact.